Researcher at European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)
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Characterising Complex Enzyme Reaction Data
Handan Melike Dönertaş
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Sergio MartĂnez Cuesta
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Syed Asad Rahman
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Janet Thornton
PLOS ONE, 2016-02-03
Video Abstract (AI generated)PaperPreprintPLOS ONE
The relationship between enzyme-catalysed reactions and the Enzyme Commission (EC) number, the widely accepted classification scheme used to characterise enzyme activity, is complex and with the rapid increase in our knowledge of the reactions catalysed by enzymes needs revisiting. We present a manual and computational analysis to investigate this complexity and found that almost one-third of all known EC numbers are linked to more than one reaction in the secondary reaction databases (e.g. KEGG). Although this complexity is often resolved by defining generic, alternative and partial reactions, we have also found individual EC numbers with more than one reaction catalysing different types of bond changes. This analysis adds a new dimension to our understanding of enzyme function and might be useful for the accurate annotation of the function of enzymes and to study the changes in enzyme function during evolution.