Author(s)
Olivia Stovicek
Published 4 Projects
Immunology Microbiology Infectious Diseases Influenza Hemagglutinin Stalk
Jenna J. Guthmiller
Published 6 Projects
Immunology Microbiology Infectious Diseases Influenza Hemagglutinin Stalk
Siriruk Changrob
Published 3 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Haley L. Dugan
Published 5 Projects
Immunology Microbiology Infectious Diseases Influenza Hemagglutinin Stalk
Christopher T. Stamper
Published 3 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Nai-Ying Zheng
Published 4 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Min Huang
Published 5 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Content
Video Abstract (AI generated) (01:32) Paper Preprint SupplementaryData (wilsonimmunologylab,github)Artificial mutagenesis and chimeric/mosaic protein engineering have laid the foundation for antigenic characterization and universal vaccine design for influenza viruses. However, many methods used for influenza research and vaccine development require sequence editing and protein expression, limiting their applicability and the progress of related research to specialists. Rapid tools allowing even novice influenza researchers to properly analyze and visualize influenza protein sequences with accurate nomenclature are needed to expand the research field. To address this need, we developed Librator, a system for analyzing and designing protein sequences of influenza virus Hemagglutinin (HA) and Neuraminidase (NA). With the graphical user interface (GUI) and built-in sequence editing functions of Librator, biologists can easily analyze influenza sequences and phylogenies, automatically port sequences to visualize structures, then readily mutate target residues and design sequences for antigen probes and chimeric/mosaic proteins efficiently and accurately. This system provides optimized fragment design for Gibson Assembly of HA and NA expression constructs based on peptide conservation of all historical HA and NA sequences, ensuring fragments are reusable and compatible, allowing for significant reagent savings. Use of Librator will significantly facilitate influenza research and vaccine antigen design.
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Patrick Wilson. (2021, Oct 29).Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins[Video]. Scitok. https://scitok.com/project/p/0cf7c0dc
Li Lei. "Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins" Scitok, uploaded by Wilson Patrick, 29 Oct, 2021, https://scitok.com/project/p0cf7c0dc
Patrick Wilson. "Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins" Scitok. (Oct 29, 2021). https://scitok.com/project/p/0cf7c0dc
Patrick Wilson (Oct 29, 2021). Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins Scitok. https://scitok.com/project/p/0cf7c0dc
Patrick Wilson. Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins[video]. 2021 Oct 29. https://scitok.com/project/p/0cf7c0dc
@online{al2006link, title={ Librator, a platform for optimized sequence editing, design, and expression of influenza virus proteins }, author={ Wilson, Patrick }, organization={Scitok}, month={ Oct }, day={ 29 }, year={ 2021 }, url = {https://scitok.com/project/p/0cf7c0dc}, }