Author(s)
Zhuoming Liu
Published 14 Projects
Infectious Diseases Cell Biology Inhibition COVID-19 Vacuolin 1
Sean P. J. Whelan
Published 14 Projects
Infectious Diseases Cell Biology Inhibition COVID-19 Vacuolin 1
Content
Cholesterol 25-hydroxylase (CH25H) is an interferon-stimulated gene (ISG) that shows broad antiviral activities against a wide range of enveloped viruses. Here, using an ISG screen against VSV-SARS-CoV and VSV-SARS-CoV-2 chimeric viruses, we identified CH25H and its enzymatic product 25-hydroxycholesterol (25HC) as potent inhibitors of virus replication. Mechanistically, internalized 25HC accumulates in the late endosomes and blocks cholesterol export, thereby restricting SARS-CoV-2 spike protein catalyzed membrane fusion. Our results highlight a unique antiviral mechanism of 25HC and provide the molecular basis for its possible therapeutic development. ### Competing Interest Statement M.S.D. is a consultant for Inbios, Eli Lilly, Vir Biotechnology, NGM Biopharmaceuticals, and Emergent BioSolutions and on the Scientific Advisory Board of Moderna. The Diamond laboratory at Washington University School of Medicine has received sponsored research agreements from Moderna. Invention disclosure filed with Washington University in St. Louis for the recombinant VSV-SARS-CoV-2 used herein.
More Projects
Loren Frank
13 views • 2 years ago
Global Immunotalks
390 views • 2 years ago
Laurel Yohe
2 views • 2 years ago
Global Immunotalks
130 views • 2 years ago
Jignesh H. Parmar
0 views • 2 years ago
Winston A. Haynes
0 views • 2 years ago
Noam Mazor
0 views • 2 years ago
Global Immunotalks
182 views • 2 years ago
Cem Yuksel
345 views • 2 years ago
Oscar Gonzalez-Recio
3 views • 2 years ago
Please pick a style:
Zhuoming Liu. (2021, Nov 6).Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion[Video]. Scitok. https://scitok.com/project/p/27939071
Zang Ruochen. "Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion" Scitok, uploaded by Liu Zhuoming, 6 Nov, 2021, https://scitok.com/project/p27939071
Zhuoming Liu. "Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion" Scitok. (Nov 6, 2021). https://scitok.com/project/p/27939071
Zhuoming Liu (Nov 6, 2021). Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion Scitok. https://scitok.com/project/p/27939071
Zhuoming Liu. Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion[video]. 2021 Nov 6. https://scitok.com/project/p/27939071
@online{al2006link, title={ Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion }, author={ Liu, Zhuoming }, organization={Scitok}, month={ Nov }, day={ 6 }, year={ 2021 }, url = {https://scitok.com/project/p/27939071}, }