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Video Abstract (AI generated) (01:26) Paper Preprint Expanded View Figure 1 Expanded View Figure 2 Expanded View Figure 3 Expanded View Figure 4 Expanded View Figure 5 Expanded View Figure 6ESX-1 is a major virulence factor of Mycobacterium tuberculosis, a secretion machinery directly involved in the survival of the microorganism from the immune system defence. It disrupts the phagosome membrane of the host cell through a contact-dependent mechanism. Recently, the structure of the inner-membrane core complex of the homologous ESX-3 and ESX-5 was resolved; however, the elements involved in the secretion through the outer membrane or those acting on the host cell membrane are unknown. Protein substrates might form this missing element. Here, we describe the oligomerisation process of the ESX-1 substrate EspB, which occurs upon cleavage of its C-terminal region and is favoured by an acidic environment. Cryo-electron microscopy data are presented which show that EspB from different mycobacterial species have a conserved quaternary structure, except for the non-pathogenic species M. smegmatis. EspB assembles into a channel with dimensions and characteristics suitable for the transit of ESX-1 substrates, as shown by the presence of another EspB trapped within. Our results provide insight into the structure and assembly of EspB, and suggests a possible function as a structural element of ESX-1.
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Abril Gijsbers. (2021, Oct 31).Priming mycobacterial ESX-secreted protein B to form a channel-like structure[Video]. Scitok. https://scitok.com/project/p/27b2d6cc
Gijsbers Abril. "Priming mycobacterial ESX-secreted protein B to form a channel-like structure" Scitok, uploaded by Gijsbers Abril, 31 Oct, 2021, https://scitok.com/project/p27b2d6cc
Abril Gijsbers. "Priming mycobacterial ESX-secreted protein B to form a channel-like structure" Scitok. (Oct 31, 2021). https://scitok.com/project/p/27b2d6cc
Abril Gijsbers (Oct 31, 2021). Priming mycobacterial ESX-secreted protein B to form a channel-like structure Scitok. https://scitok.com/project/p/27b2d6cc
Abril Gijsbers. Priming mycobacterial ESX-secreted protein B to form a channel-like structure[video]. 2021 Oct 31. https://scitok.com/project/p/27b2d6cc
@online{al2006link, title={ Priming mycobacterial ESX-secreted protein B to form a channel-like structure }, author={ Gijsbers, Abril }, organization={Scitok}, month={ Oct }, day={ 31 }, year={ 2021 }, url = {https://scitok.com/project/p/27b2d6cc}, }