Author(s)
Angelique R. Ormsby
Published 3 Projects
Biochemistry Cell Biology Protein Aggregation Methylosome Abce1
Danny Hatters
Published 4 Projects
Metabolism Biochemistry Cell Biology Protein Aggregation Proteasome
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Video Abstract (AI generated) (01:37) Paper Preprint PLOS ONEMutations that cause Huntington’s Disease involve a polyglutamine (polyQ) sequence expansion beyond 35 repeats in exon 1 of Huntingtin. Intracellular inclusion bodies of mutant Huntingtin protein are a key feature of Huntington’s disease brain pathology. We previously showed that in cell culture the formation of inclusions involved the assembly of disordered structures of mHtt exon 1 fragments (Httex1) and they were enriched with translational machinery when first formed. We hypothesized that nascent mutant Httex1 chains co-aggregate during translation by phase separation into liquid-like disordered aggregates and then convert to more rigid, amyloid structures. Here we further examined the mechanisms of inclusion assembly in a human epithelial kidney (AD293) cell culture model and examined whether ribosome quality control machinery previously implicated in stalled ribosomes were involved. We found mHttex1 did not appear to stall translation of its own nascent chain and there was no recruitment of RNA into inclusions. However, proteins involved in translation or ribosome quality control were co-recruited into the inclusions (Ltn1 and Rack1) compared to a protein not anticipated to be involved (NACAD). Furthermore, we observed co-aggregation with other proteins previously identified in inclusions, including Upf-1 and chaperone-like proteins Sgta and Hspb1, which also suppressed aggregation at high co-expression levels. The newly formed inclusions contained immobile mHttex1 molecules which points to the disordered aggregates being mechanically rigid prior to amyloid formation.
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Danny Hatters. (2021, Nov 1).Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control[Video]. Scitok. https://scitok.com/project/p/3e2c79b8
R. Ormsby Angelique. "Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control" Scitok, uploaded by Hatters Danny, 1 Nov, 2021, https://scitok.com/project/p3e2c79b8
Danny Hatters. "Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control" Scitok. (Nov 1, 2021). https://scitok.com/project/p/3e2c79b8
Danny Hatters (Nov 1, 2021). Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control Scitok. https://scitok.com/project/p/3e2c79b8
Danny Hatters. Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control[video]. 2021 Nov 1. https://scitok.com/project/p/3e2c79b8
@online{al2006link, title={ Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control }, author={ Hatters, Danny }, organization={Scitok}, month={ Nov }, day={ 1 }, year={ 2021 }, url = {https://scitok.com/project/p/3e2c79b8}, }