Author(s)
Linda Yu-Ling Lan
Published 2 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Min Huang
Published 5 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Carole Henry
Published 5 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Content
Video Abstract (AI generated) (01:28) Paper Preprint Journal of VirologyHemagglutinin (HA) stalk-reactive antibodies are the basis of several current one-shot universal influenza vaccine efforts because they protect against a wide spectrum of influenza virus strains. The appreciated mechanism of protection by HA-stalk antibodies is to inhibit HA stalk reconfiguration, blocking viral fusion and entry. This study shows that HA stalk-reactive antibodies also inhibit neuraminidase (NA) enzymatic activity, prohibiting viral egress. NA inhibition (NI) is evident for an attached substrate but not for unattached small molecule cleavage of sialic acid. This suggests that the antibodies inhibit NA enzymatic activity through steric hindrance, thus limiting NA access to sialic acids when adjacent to HA on whole virions. Consistently, F(ab)2 fragments that occupy reduced area without loss of avidity or disrupted HA/NA interactions show significantly reduced NI activity. Notably, HA stalk binding antibodies lacking NI activity were unable to neutralize viral infection via microneutralization assays. This work suggests that NI activity is an important component of HA-stalk antibody mediated protection.
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Patrick Wilson. (2021, Oct 29).Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance[Video]. Scitok. https://scitok.com/project/p/4b1933c5
Chen Yao-Qing. "Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance" Scitok, uploaded by Wilson Patrick, 29 Oct, 2021, https://scitok.com/project/p4b1933c5
Patrick Wilson. "Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance" Scitok. (Oct 29, 2021). https://scitok.com/project/p/4b1933c5
Patrick Wilson (Oct 29, 2021). Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance Scitok. https://scitok.com/project/p/4b1933c5
Patrick Wilson. Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance[video]. 2021 Oct 29. https://scitok.com/project/p/4b1933c5
@online{al2006link, title={ Hemagglutinin stalk-reactive antibodies interfere with influenza virus neuraminidase activity by steric hindrance }, author={ Wilson, Patrick }, organization={Scitok}, month={ Oct }, day={ 29 }, year={ 2021 }, url = {https://scitok.com/project/p/4b1933c5}, }