Author(s)
Giulia Menchinelli
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Licia Bordi
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Flora Marzialiotti
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Ivana Palucci
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Maria R. Capobianchi
Published 5 Projects
Immunology SARS-CoV-2 Bioinformatics Biochemistry Infectious Diseases
Giuseppe Sberna
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Eleonora Lalle
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Lucio Romano
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Giulia De Angelis
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Simona Marchetti
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Maurizio Sanguinetti
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Paola Cattani
Published 1 Project
SARS-CoV-2 Infectious Diseases Testing Keywords Antigen Detection Lumipulse Assay
Content
Compared to RT-PCR, lower performance of antigen detection assays, including the Lumipulse G SARS-CoV-2 Ag assay, may depend on specific testing scenarios. We tested 594 nasopharyngeal swab samples from individuals with COVID-19 (RT-PCR cycle threshold [Ct] values [≤]40) or non-COVID-19 (Ct values [≤]40) diagnoses. RT-PCR positive samples were assigned to diagnostic, screening, or monitoring groups of testing. With a limit of detection of 1.2 x 104 SARS-CoV-2 RNA copies/ml, Lumipulse showed positive percent agreement (PPA) of 79.9% (155/194) and negative percent agreement of 99.3% (397/400), whereas PPAs were 100% for samples with Ct values of <18 or 18-<25 and 92.5% for samples with Ct values of 25-<30. By three groups, Lumipulse showed PPA of 87.0% (60/69), 81.1% (43/53), or 72.2% (52/72), respectively, whereas PPA was 100% for samples with Ct values of <18 or 18-<25, and was 94.4%, 80.0%, or 100% for samples with Ct values of 25-<30, respectively. RT-PCR positive samples were also tested for SARS-CoV-2 subgenomic RNA and, by three groups, testing showed that PPA was 63.8% (44/69), 62.3% (33/53), or 33.3% (24/72), respectively. PPAs dropped to 55.6%, 20.0%, or 41.7% for samples with Ct values of 25-<30, respectively. All 101 samples with a subgenomic RNA positive result had a Lumipulse assays antigen positive result, whereas only 54 (58.1%) of remaining 93 samples had a Lumipulse assays antigen positive result. In conclusion, Lumipulse assay was highly sensitive in samples with low RT-PCR Ct values, implying repeated testing to reduce consequences of false-negative results.
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Maria R. Capobianchi. (2021, Oct 23).Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups[Video]. Scitok. https://scitok.com/project/p/51410e2b
Menchinelli Giulia. "Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups" Scitok, uploaded by R. Capobianchi Maria, 23 Oct, 2021, https://scitok.com/project/p51410e2b
Maria R. Capobianchi. "Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups" Scitok. (Oct 23, 2021). https://scitok.com/project/p/51410e2b
Maria R. Capobianchi (Oct 23, 2021). Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups Scitok. https://scitok.com/project/p/51410e2b
Maria R. Capobianchi. Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups[video]. 2021 Oct 23. https://scitok.com/project/p/51410e2b
@online{al2006link, title={ Lumipulse G SARS-CoV-2 Ag Assay Evaluation for SARS-CoV-2 Antigen Detection Using 594 Nasopharyngeal Swab Samples from Different Testing Groups }, author={ R. Capobianchi, Maria }, organization={Scitok}, month={ Oct }, day={ 23 }, year={ 2021 }, url = {https://scitok.com/project/p/51410e2b}, }