Author(s)
Anuradha Vajjala
Published 1 Project
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Debabrata Biswas
Published 1 Project
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Kelvin Kian Long Chong
Published 5 Projects
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Wei Hong Tay
Published 2 Projects
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Emanuel Hanski
Published 1 Project
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Kimberly A. Kline
Published 5 Projects
Microbiology Necrotizing Fasciitis Biofilm Er Stress Streptolysins
Content
Video Abstract (AI generated) (02:02) Paper PreprintGroup A Streptococcus (GAS) is a human pathogen that causes infections ranging from mild to fulminant and life-threatening. Biofilms have been implicated in acute GAS soft-tissue infections such as necrotizing fasciitis (NF). However, most in vitro models used to study GAS biofilms have been designed to mimic chronic infections and insufficiently recapitulate in vivo conditions and the host-pathogen interactions that might influence biofilm formation. Here we establish and characterize an in vitro model of GAS biofilm development on mammalian cells that simulates microcolony formation observed in a murine model of human NF. We show that on mammalian cells, GAS forms dense aggregates that display hallmark biofilm characteristics including a three-dimensional architecture and enhanced tolerance to antibiotics. In contrast to abiotic-grown biofilms, host-associated biofilms require the expression of secreted GAS streptolysins O and S (SLO, SLS) resulting in the release of a host-associated biofilm promoting-factor(s). Supernatants from GAS-infected mammalian cells or from cells treated with endoplasmic reticulum (ER) stressors restore biofilm formation to an SLO and SLS null mutant that is otherwise attenuated in biofilm formation on cells, together suggesting a role for streptolysin-induced ER stress in this process. In an in vivo mouse model, the streptolysin-null mutant is attenuated in both microcolony formation and bacterial spread, but pre-treatment of soft-tissue with an ER-stressor restores the ability of the mutant to form wild type like microcolonies that disseminate throughout the soft tissue. Taken together, we have identified a new role of streptolysin-driven ER stress in GAS biofilm formation and NF disease progression.
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Kelvin Kian Long Chong. (2021, Nov 5).Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis[Video]. Scitok. https://scitok.com/project/p/531cc7ed
Vajjala Anuradha. "Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis" Scitok, uploaded by Kian Long Chong Kelvin, 5 Nov, 2021, https://scitok.com/project/p531cc7ed
Kelvin Kian Long Chong. "Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis" Scitok. (Nov 5, 2021). https://scitok.com/project/p/531cc7ed
Kelvin Kian Long Chong (Nov 5, 2021). Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis Scitok. https://scitok.com/project/p/531cc7ed
Kelvin Kian Long Chong. Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis[video]. 2021 Nov 5. https://scitok.com/project/p/531cc7ed
@online{al2006link, title={ Streptolysin-induced endoplasmic reticulum stress promotes group A streptococcal in vivo biofilm formation and necrotizing fasciitis }, author={ Kian Long Chong, Kelvin }, organization={Scitok}, month={ Nov }, day={ 5 }, year={ 2021 }, url = {https://scitok.com/project/p/531cc7ed}, }