Author(s)
Loren Frank
Professor at University of California, San Francisco
Field of Study: Biology , Published 30 Projects
Animal Behavior And Cognition Real Time Sharp Wave Ripple Theta Oscillations Public Speaking
Content
Video Abstract (AI generated) (01:20) Paper Preprint Video 1Anatomic evaluation is an important aspect of many studies in neuroscience; however, it often lacks information about the three-dimensional structure of the brain. Micro-CT imaging provides an excellent, nondestructive, method for the evaluation of brain structure, but current applications to neurophysiological or lesions studies require removal of the skull and hazardous chemicals, dehydration or embedding, limiting their scalability and utility. Here we present a protocol using eosin in combination with bone decalcification to enhance contrast in the tissue and then employ monochromatic and propagation phase-contrast micro-CT imaging to enable the imaging of brain structure with the preservation of the surrounding skull. Instead of relying on descriptive, time-consuming, or subjective methods, we develop simple quantitative analyses to map the locations of recording electrodes and to characterize the presence and extent of brain lesions. ### Competing Interest Statement VK is employed (part-time) by Neuralink Corp. The other authors declare no conflicts of interest.
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Loren Frank. (2021, Nov 7).Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants[Video]. Scitok. https://scitok.com/project/p/6cc01821
B. Kastner David. "Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants" Scitok, uploaded by Frank Loren, 7 Nov, 2021, https://scitok.com/project/p6cc01821
Loren Frank. "Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants" Scitok. (Nov 7, 2021). https://scitok.com/project/p/6cc01821
Loren Frank (Nov 7, 2021). Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants Scitok. https://scitok.com/project/p/6cc01821
Loren Frank. Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants[video]. 2021 Nov 7. https://scitok.com/project/p/6cc01821
@online{al2006link, title={ Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants }, author={ Frank, Loren }, organization={Scitok}, month={ Nov }, day={ 7 }, year={ 2021 }, url = {https://scitok.com/project/p/6cc01821}, }