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Video Abstract (AI generated) (01:27) Paper PreprintFrequent outbreaks of novel coronaviruses (CoVs), highlighted by the current SARS-CoV-2 pandemic, necessitate the development of therapeutics that could be easily and effectively administered world-wide. The conserved mRNA-capping process enables CoVs to evade their host immune system and is a target for antiviral development. Nonstructural protein (nsp) 16 in complex with nsp10 catalyzes the final step of coronaviral mRNA-capping through its 2'-O-methylation activity. Like other methyltransferases, SARS-CoV-2 nsp10-nsp16 complex is druggable. However, the availability of an optimized assay for high-throughput screening (HTS) is an unmet need. Here, we report the development of a radioactivity-based assay for methyltransferase activity of nsp10-nsp16 complex in a 384-well format, and kinetic characterization, and optimization of the assay for HTS (Z'-factor: 0.83). Considering the high conservation of nsp16 across known CoV species, the potential inhibitors targeting SARS-CoV-2 nsp10-nsp16 complex may also be effective against other emerging pathogenic CoVs.
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Fengling Li. (2021, Sep 30).A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex[Video]. Scitok. https://scitok.com/project/p/88db4837
Khalili Yazdi Aliakbar. "A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex" Scitok, uploaded by Li Fengling, 30 Sep, 2021, https://scitok.com/project/p88db4837
Fengling Li. "A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex" Scitok. (Sep 30, 2021). https://scitok.com/project/p/88db4837
Fengling Li (Sep 30, 2021). A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex Scitok. https://scitok.com/project/p/88db4837
Fengling Li. A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex[video]. 2021 Sep 30. https://scitok.com/project/p/88db4837
@online{al2006link, title={ A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex }, author={ Li, Fengling }, organization={Scitok}, month={ Sep }, day={ 30 }, year={ 2021 }, url = {https://scitok.com/project/p/88db4837}, }