Author(s)
Alexander J Mentzer
Group Leader, Wellcome Centre for Human Genetics, University of Oxford
Published 2 Projects
Content
Video Abstract (AI generated) (01:31) Paper PreprintA major issue in identification of protective T cell responses against SARS-CoV-2 lies in distinguishing people infected with SARS-CoV-2 from those with cross-reactive immunity generated by exposure to other coronaviruses. We characterised SARS-CoV-2 T cell immune responses in 168 PCR-confirmed SARS-CoV-2 infected subjects and 118 seronegative subjects without known SARS-CoV-2 exposure using a range of T cell assays that differentially capture immune cell function. Strong ex vivo ELISpot and proliferation responses to multiple antigens (including M, NP and ORF3) were found in those who had been infected by SARS-CoV-2 but were rare in pre-pandemic and unexposed seronegative subjects. However, seronegative doctors with high occupational exposure and recent COVID-19 compatible illness showed patterns of T cell responses characteristic of infection, indicating that these readouts are highly sensitive. By contrast, over 90% of convalescent or unexposed people showed proliferation and cellular lactate responses to spike subunits S1/S2, indicating pre-existing cross-reactive T cell populations. The detection of T cell responses to SARS-CoV-2 is therefore critically dependent on the choice of assay and antigen. Memory responses to specific non-spike proteins provides a method to distinguish recent infection from pre-existing immunity in exposed populations.
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Narayan Ramamurthy. (2021, Sep 29).T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses[Video]. Scitok. https://scitok.com/project/p/8a40ee1c
Ogbe Ane. "T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses" Scitok, uploaded by Ramamurthy Narayan, 29 Sep, 2021, https://scitok.com/project/p8a40ee1c
Narayan Ramamurthy. "T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses" Scitok. (Sep 29, 2021). https://scitok.com/project/p/8a40ee1c
Narayan Ramamurthy (Sep 29, 2021). T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses Scitok. https://scitok.com/project/p/8a40ee1c
Narayan Ramamurthy. T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses[video]. 2021 Sep 29. https://scitok.com/project/p/8a40ee1c
@online{al2006link, title={ T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses }, author={ Ramamurthy, Narayan }, organization={Scitok}, month={ Sep }, day={ 29 }, year={ 2021 }, url = {https://scitok.com/project/p/8a40ee1c}, }