Author(s)
Junhong Choi
Published 5 Projects
Biochemistry Molecular Biology Bioengineering Translation Ribosome
Arjun Prabhakar
Published 2 Projects
Biochemistry Translation Ribosome Translocation Single Molecule Fret
Alexey Petrov
Published 2 Projects
Biochemistry Translation Ribosome Translocation Single Molecule Fret
Joseph Puglisi
Published 4 Projects
Biochemistry Molecular Biology Translation Ribosome Translocation
Content
Video Abstract (AI generated) (01:40) Paper Preprint NatureTranslation initiation determines both the quantity and identity of the protein product by establishing the reading frame for protein synthesis. In eukaryotic cells, numerous translation initiation factors (eIFs) prepare ribosomes for polypeptide elongation, yet the underlying dynamics of this process remain enigmatic. A central question is how eukaryotic ribosomes transition from translation initiation to elongation. Here, we applied in vitro single-molecule fluorescence microscopy approaches to monitor directly in real time the pathways of late translation initiation and the transition to elongation using a purified yeast Saccharomyces cerevisiae translation system. This transition was remarkably slower in our eukaryotic system than that reported for Escherichia coli. The slow entry to elongation was defined by a long residence time of eIF5B on the 80S ribosome after joining of individual ribosomal subunits, which is catalyzed by this universally conserved initiation factor. Inhibition of eIF5B GTPase activity following subunit joining prevented eIF5B dissociation from the 80S complex, thereby preventing elongation. Our findings illustrate how eIF5B dissociation serves as a kinetic checkpoint for the transition from initiation to elongation, and its release may be governed by a conformation of the ribosome complex that triggers GTP hydrolysis.
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Junhong Choi. (2021, Nov 4).eIF5B gates the transition from translation initiation to elongation[Video]. Scitok. https://scitok.com/project/p/99c7b44f
Wang Jinfan. "eIF5B gates the transition from translation initiation to elongation" Scitok, uploaded by Choi Junhong, 4 Nov, 2021, https://scitok.com/project/p99c7b44f
Junhong Choi. "eIF5B gates the transition from translation initiation to elongation" Scitok. (Nov 4, 2021). https://scitok.com/project/p/99c7b44f
Junhong Choi (Nov 4, 2021). eIF5B gates the transition from translation initiation to elongation Scitok. https://scitok.com/project/p/99c7b44f
Junhong Choi. eIF5B gates the transition from translation initiation to elongation[video]. 2021 Nov 4. https://scitok.com/project/p/99c7b44f
@online{al2006link, title={ eIF5B gates the transition from translation initiation to elongation }, author={ Choi, Junhong }, organization={Scitok}, month={ Nov }, day={ 4 }, year={ 2021 }, url = {https://scitok.com/project/p/99c7b44f}, }