Author(s)
Natalia A. Bulgakova
Published 5 Projects
Cell Biology Developmental Biology Endocytosis Rhoa E Cadherin
Content
Video Abstract (AI generated) (01:33) Paper PreprintThe regulation of E-cadherin at the plasma membrane by endocytosis is of vital importance for developmental and disease. p120-catenin, which binds to the E-cadherin C-terminus, can both promote and inhibit E-cadherin endocytosis. However, little is known about what determines the directionality of p120-catenin activity, and the molecules downstream. Here, we have discovered that p120-catenin fine-tunes the clathrin-mediated endocytosis of E-cadherin in Drosophila embryonic epidermal cells. It simultaneously activated two actin-remodelling pathways with opposing effects: RhoA, which stabilized E-cadherin at the membrane, and Arf1, which promoted internalization. Epistasis experiments revealed that RhoA additionally inhibited Arf1. E-cadherin was efficiently endocytosed only in the presence of intermediate p120-catenin amounts with too little and too much p120-catenin inhibiting E-cadherin endocytosis. Finally, we found that p120-catenin levels altered the tension of the plasma membrane. Altogether, this shows that p120-catenin is a central hub which co-ordinates cell adhesion, endocytosis, and actin dynamics with tissue tension.
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Danny Reinberg. (2021, Nov 7).E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1[Video]. Scitok. https://scitok.com/project/p/a5c1b05e
Greig Joshua. "E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1" Scitok, uploaded by Reinberg Danny, 7 Nov, 2021, https://scitok.com/project/pa5c1b05e
Danny Reinberg. "E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1" Scitok. (Nov 7, 2021). https://scitok.com/project/p/a5c1b05e
Danny Reinberg (Nov 7, 2021). E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1 Scitok. https://scitok.com/project/p/a5c1b05e
Danny Reinberg. E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1[video]. 2021 Nov 7. https://scitok.com/project/p/a5c1b05e
@online{al2006link, title={ E-cadherin endocytosis is modulated by p120-catenin through the opposing actions of RhoA and Arf1 }, author={ Reinberg, Danny }, organization={Scitok}, month={ Nov }, day={ 7 }, year={ 2021 }, url = {https://scitok.com/project/p/a5c1b05e}, }