Author(s)
Content
Video Abstract (AI generated) (01:31) Paper Preprint Supplemental Table 1 Supplemental Table 2 Supplemental Table 3 Supplemental Table 4 Supplemental Table 5 Supplemental Table 6 Supplemental Table 7 Supplemental Table 8 (assets,nemoarchive) (nemoanalytics,"") (brainome,ucsd)Single cell transcriptomics has transformed the characterization of brain cell identity by providing quantitative molecular signatures for large, unbiased samples of brain cell populations. With the proliferation of taxonomies based on individual datasets, a major challenge is to integrate and validate results toward defining biologically meaningful cell types. We used a battery of single-cell transcriptome and epigenome measurements generated by the BRAIN Initiative Cell Census Network (BICCN) to comprehensively assess the molecular signatures of cell types in the mouse primary motor cortex (MOp). We further developed computational and statistical methods to integrate these multimodal data and quantitatively validate the reproducibility of the cell types. The reference atlas, based on more than 600,000 high quality single-cell or -nucleus samples assayed by six molecular modalities, is a comprehensive molecular account of the diverse neuronal and non-neuronal cell types in MOp. Collectively, our study indicates that the mouse primary motor cortex contains over 55 neuronal cell types that are highly replicable across analysis methods, sequencing technologies, and modalities. We find many concordant multimodal markers for each cell type, as well as thousands of genes and gene regulatory elements with discrepant transcriptomic and epigenomic signatures. These data highlight the complex molecular regulation of brain cell types and will directly enable design of reagents to target specific MOp cell types for functional analysis.
More Projects
Loren Frank
13 views • 2 years ago
Global Immunotalks
390 views • 3 years ago
Laurel Yohe
2 views • 2 years ago
Global Immunotalks
130 views • 3 years ago
Jignesh H. Parmar
0 views • 2 years ago
Winston A. Haynes
0 views • 2 years ago
Noam Mazor
0 views • 2 years ago
Global Immunotalks
182 views • 3 years ago
Cem Yuksel
345 views • 2 years ago
Oscar Gonzalez-Recio
3 views • 2 years ago
Please pick a style:
Eeshit Dhaval Vaishnav. (2021, Nov 6).An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types[Video]. Scitok. https://scitok.com/project/p/ace330ab
Yao Zizhen. "An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types" Scitok, uploaded by Dhaval Vaishnav Eeshit, 6 Nov, 2021, https://scitok.com/project/pace330ab
Eeshit Dhaval Vaishnav. "An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types" Scitok. (Nov 6, 2021). https://scitok.com/project/p/ace330ab
Eeshit Dhaval Vaishnav (Nov 6, 2021). An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types Scitok. https://scitok.com/project/p/ace330ab
Eeshit Dhaval Vaishnav. An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types[video]. 2021 Nov 6. https://scitok.com/project/p/ace330ab
@online{al2006link, title={ An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types }, author={ Dhaval Vaishnav, Eeshit }, organization={Scitok}, month={ Nov }, day={ 6 }, year={ 2021 }, url = {https://scitok.com/project/p/ace330ab}, }