Author(s)
Tianhan Huang
Published 1 Project
Biochemistry Translation Ribosome Translocation Single Molecule Fret
Junhong Choi
Published 5 Projects
Biochemistry Molecular Biology Bioengineering Translation Ribosome
Arjun Prabhakar
Published 2 Projects
Biochemistry Translation Ribosome Translocation Single Molecule Fret
Joseph Puglisi
Published 4 Projects
Biochemistry Molecular Biology Translation Ribosome Translocation
Alexey Petrov
Published 2 Projects
Biochemistry Translation Ribosome Translocation Single Molecule Fret
Content
Video Abstract (AI generated) (01:31) Paper PreprintThe ribosome is a molecular machine that adopts at least two global states during translation. Two main steps of translation, peptidyl transfer and translocation, are accompanied by counterclockwise and clockwise rotations of the two ribosomal subunits. However, when and why the ribosome alternates between these states remains unclear, with two well supported but conflicting hypotheses. Ribosomes may undergo a single cycle of forward and backward rotations per codon read. Alternatively, in addition to rotations caused by peptidyl transfer and translocation, ribosomes may undergo multiple full spontaneous rotations, with these rotations playing a critical role in elongation and specifically in translocation mechanism. We applied high-speed single-molecule TIRF microscopy to follow translation in real-time. Actively translating ribosomes undergo partial spontaneous rotations between three different rotational states. Spontaneous rotations are restricted prior to A-site tRNA decoding. Peptidyl transfer unlocks spontaneous rotations. Consequently, translocation proceeds via a novel rotational state induced by EF-G. Our results bridge both models and provide a coherent view of ribosome dynamics during translation.
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Junhong Choi. (2021, Nov 4).Partial spontaneous intersubunit rotations in actively translating ribosomes[Video]. Scitok. https://scitok.com/project/p/ad18e03f
Huang Tianhan. "Partial spontaneous intersubunit rotations in actively translating ribosomes" Scitok, uploaded by Choi Junhong, 4 Nov, 2021, https://scitok.com/project/pad18e03f
Junhong Choi. "Partial spontaneous intersubunit rotations in actively translating ribosomes" Scitok. (Nov 4, 2021). https://scitok.com/project/p/ad18e03f
Junhong Choi (Nov 4, 2021). Partial spontaneous intersubunit rotations in actively translating ribosomes Scitok. https://scitok.com/project/p/ad18e03f
Junhong Choi. Partial spontaneous intersubunit rotations in actively translating ribosomes[video]. 2021 Nov 4. https://scitok.com/project/p/ad18e03f
@online{al2006link, title={ Partial spontaneous intersubunit rotations in actively translating ribosomes }, author={ Choi, Junhong }, organization={Scitok}, month={ Nov }, day={ 4 }, year={ 2021 }, url = {https://scitok.com/project/p/ad18e03f}, }