Author(s)
Russ Altman
Published 17 Projects
Through Manual Identification And Other In The United States In This Paper Singapore
Content
Video Abstract (AI generated) (02:37) Paper Preprint Supplementary Information/Appendix Supplementary Dataset 1 Supplementary Dataset 2 Supplementary Dataset 3 Supplementary Dataset 4 Supplementary Dataset 5As of early March, 2019-nCoV has infected more than one hundred thousand people and claimed thousands of lives. 2019-nCoV is a novel form of coronavirus that causes COVID-19 and has high similarity with SARS-CoV. No approved vaccine yet exists for any form of coronavirus. Here we use computational tools from structural biology and machine learning to identify 2019-nCoV T-cell and B-cell epitopes based on viral protein antigen presentation and antibody binding properties. These epitopes can be used to develop more effective vaccines and identify neutralizing antibodies. We identified 405 viral peptides with good antigen presentation scores for both human MHC-I and MHC-II alleles, and two potential neutralizing B-cell epitopes near the 2019-nCoV spike protein receptor binding domain (440-460 and 494-506). Analyzing mutation profiles of 68 viral genomes from four continents, we identified 96 coding-change mutations. These mutations are more likely to occur in regions with good MHC-I presentation scores (p=0.02). No mutations are present near the spike protein receptor binding domain. Based on these findings, the spike protein is likely immunogenic and a potential vaccine candidate. We validated our computational pipeline with SARS-CoV experimental data. Significance Statement The novel coronavirus 2019-nCoV has affected more than 100 countries and continues to spread. There is an immediate need for effective vaccines that contain antigens which trigger responses from human T-cells and B-cells (known as epitopes). Here we identify potential T-cell epitopes through an analysis of human antigen presentation, as well as B-cell epitopes through an analysis of protein structure. We identify a list of top candidates, including an epitope located on 2019-nCoV spike protein that potentially triggers both T-cell and B-cell responses. Analyzing 68 samples, we observe that viral mutations are more likely to happen in regions with strong antigen presentation, a potential form of immune evasion. Our computational pipeline is validated with experimental data from SARS-CoV.
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Russ Altman. (2021, Oct 26).Potential T-cell and B-cell Epitopes of 2019-nCoV[Video]. Scitok. https://scitok.com/project/p/b21deaef
Fast Ethan. "Potential T-cell and B-cell Epitopes of 2019-nCoV" Scitok, uploaded by Altman Russ, 26 Oct, 2021, https://scitok.com/project/pb21deaef
Russ Altman. "Potential T-cell and B-cell Epitopes of 2019-nCoV" Scitok. (Oct 26, 2021). https://scitok.com/project/p/b21deaef
Russ Altman (Oct 26, 2021). Potential T-cell and B-cell Epitopes of 2019-nCoV Scitok. https://scitok.com/project/p/b21deaef
Russ Altman. Potential T-cell and B-cell Epitopes of 2019-nCoV[video]. 2021 Oct 26. https://scitok.com/project/p/b21deaef
@online{al2006link, title={ Potential T-cell and B-cell Epitopes of 2019-nCoV }, author={ Altman, Russ }, organization={Scitok}, month={ Oct }, day={ 26 }, year={ 2021 }, url = {https://scitok.com/project/p/b21deaef}, }