Author(s)
Begoña Monterroso
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Silvia Zorrilla
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Marta Sobrinos-Sanguino
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Miguel Ángel Robles-Ramos
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Carlos Alfonso
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Bill Söderström
Published 1 Project
Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins Division
Jolanda Verheul
Published 3 Projects
Microbiology Biochemistry Protein Membrane Interaction Keywords Bacterial Division Dna Binding Proteins
Tanneke den Blaauwen
Published 9 Projects
Microbiology Biochemistry Key Words Running Title Cell Division Divisome
Content
Video Abstract (AI generated) (01:26) Paper Preprint mBioDivision ring formation at midcell is controlled by various mechanisms in Escherichia coli, one of them being the linkage between the chromosomal Ter macrodomain and the Z-ring mediated by MatP, a DNA binding protein that organizes this macrodomain and contributes to the prevention of premature chromosome segregation. Here we show that, during cell division, just before splitting the daughter cells, MatP seems to localize close to the cytoplasmic membrane, suggesting that this protein might interact with lipids. To test this hypothesis, we investigated MatP interaction with lipids in vitro. We found that MatP, when encapsulated inside microdroplets generated by microfluidics and giant vesicles, accumulates at phospholipid bilayers and monolayers matching the lipid composition in the E. coli inner membrane. MatP binding to lipids was independently confirmed using lipid coated microbeads and bio-layer interferometry assays. Interaction of MatP with the lipid membranes also occurs in the presence of the DNA sequences specifically targeted by the protein but there is no evidence of ternary membrane/protein/DNA complexes. We propose that the interaction of MatP with lipids may modulate its spatiotemporal localization and its recognition of other ligands.
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Tanneke den Blaauwen. (2021, Nov 3).The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes[Video]. Scitok. https://scitok.com/project/p/bfcae011
Monterroso Begoña. "The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes" Scitok, uploaded by den Blaauwen Tanneke, 3 Nov, 2021, https://scitok.com/project/pbfcae011
Tanneke den Blaauwen. "The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes" Scitok. (Nov 3, 2021). https://scitok.com/project/p/bfcae011
Tanneke den Blaauwen (Nov 3, 2021). The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes Scitok. https://scitok.com/project/p/bfcae011
Tanneke den Blaauwen. The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes[video]. 2021 Nov 3. https://scitok.com/project/p/bfcae011
@online{al2006link, title={ The bacterial DNA binding protein MatP involved in linking the nucleoid terminal domain to the divisome at midcell interacts with lipid membranes }, author={ den Blaauwen, Tanneke }, organization={Scitok}, month={ Nov }, day={ 3 }, year={ 2021 }, url = {https://scitok.com/project/p/bfcae011}, }