Author(s)
F. Veronica Greco
Published 1 Project
Synthetic Biology Gene Expression Control Transcription Translation
Tobias J. Erb
Published 1 Project
Synthetic Biology Gene Expression Control Transcription Translation
Claire S Grierson
Published 2 Projects
Microscopy Synthetic Biology Gene Expression Deep Learning Control
Content
Video Abstract (AI generated) (01:32) Paper Preprint Supplementary InformationStrictly controlled inducible gene expression is crucial when engineering biological systems where even tiny amounts of a protein have a large impact on function or host cell viability. In these cases, leaky protein production must be avoided at all costs, but ideally without affecting the achievable range of expression. Here, we demonstrate how the central dogma offers a simple way to effectively address this challenge. By simultaneously regulating both transcription and translation, we show how relative basal expression of an inducible system can be greatly reduced, with minimal impact on the maximum induced expression rate. Using this approach, we create several stringent expression systems displaying >1000-fold change in their output after induction in vivo and up to a 350-fold change when used in a cell-free expression system. Furthermore, we find that multi-level regulation is able to suppress transcriptional noise and creates a digital-like switch when transitioning between 'on' and 'off' states. This work provides foundational knowledge and a genetic toolkit of parts to create multi-level gene expression controllers for those working with toxic genes or requiring precise regulation and propagation of cellular signals. It also demonstrates the value of exploring more complex and diverse regulatory designs for synthetic biology.
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Thomas E. Gorochowski. (2021, Nov 3).Harnessing the central dogma for stringent multi-level control of gene expression[Video]. Scitok. https://scitok.com/project/p/c49189fe
Veronica Greco F.. "Harnessing the central dogma for stringent multi-level control of gene expression" Scitok, uploaded by E. Gorochowski Thomas, 3 Nov, 2021, https://scitok.com/project/pc49189fe
Thomas E. Gorochowski. "Harnessing the central dogma for stringent multi-level control of gene expression" Scitok. (Nov 3, 2021). https://scitok.com/project/p/c49189fe
Thomas E. Gorochowski (Nov 3, 2021). Harnessing the central dogma for stringent multi-level control of gene expression Scitok. https://scitok.com/project/p/c49189fe
Thomas E. Gorochowski. Harnessing the central dogma for stringent multi-level control of gene expression[video]. 2021 Nov 3. https://scitok.com/project/p/c49189fe
@online{al2006link, title={ Harnessing the central dogma for stringent multi-level control of gene expression }, author={ E. Gorochowski, Thomas }, organization={Scitok}, month={ Nov }, day={ 3 }, year={ 2021 }, url = {https://scitok.com/project/p/c49189fe}, }