Author(s)
Sandra Segeletz
Published 1 Project
Cell Biology Endosomes Rab5 Multi Colour Clem Super Resolution Microscopy
Nicolas Brouilly
Published 1 Project
Cell Biology Endosomes Rab5 Multi Colour Clem Super Resolution Microscopy
Jean-Marc Verbavatz
Published 1 Project
Cell Biology Endosomes Rab5 Multi Colour Clem Super Resolution Microscopy
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Video Abstract (AI generated) (01:45) Paper Preprint TrafficMany cellular organelles, including endosomes, show compartmentalization into distinct functional domains, which however cannot be resolved by diffraction-limited light microscopy. Single molecule localization microscopy (SMLM) offers nanoscale resolution but data interpretation is often inconclusive when the ultrastructural context is missing. Correlative light electron microscopy (CLEM) combining SMLM with electron microscopy (EM) enables correlation of functional sub-domains of organelles in relation to their underlying ultrastructure at nanometer resolution. However, the specific demands for EM sample preparation and the requirements for fluorescent single-molecule photo-switching are opposed. Here, we developed a novel superCLEM workflow that combines triple-colour SMLM (dSTORM & PALM) and electron tomography using semi-thin Tokuyasu thawed cryosections. We applied the superCLEM approach to directly visualize nanoscale compartmentalization of endosomes in HeLa cells. Internalized, fluorescently labelled Transferrin and EGF were resolved into morphologically distinct domains within the same endosome. We found that the small GTPase Rab5 is organized in nano-domains on the globular part of early endosomes. The simultaneous visualization of several proteins in functionally distinct endosomal sub-compartments demonstrates the potential of superCLEM to link the ultrastructure of organelles with their molecular organization at nanoscale resolution.
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Yannis Kalaidzidis. (2021, Nov 1).Correlative SMLM and electron tomography reveals endosome nanoscale domains[Video]. Scitok. https://scitok.com/project/p/c777d4a8
Franke Christian. "Correlative SMLM and electron tomography reveals endosome nanoscale domains" Scitok, uploaded by Kalaidzidis Yannis, 1 Nov, 2021, https://scitok.com/project/pc777d4a8
Yannis Kalaidzidis. "Correlative SMLM and electron tomography reveals endosome nanoscale domains" Scitok. (Nov 1, 2021). https://scitok.com/project/p/c777d4a8
Yannis Kalaidzidis (Nov 1, 2021). Correlative SMLM and electron tomography reveals endosome nanoscale domains Scitok. https://scitok.com/project/p/c777d4a8
Yannis Kalaidzidis. Correlative SMLM and electron tomography reveals endosome nanoscale domains[video]. 2021 Nov 1. https://scitok.com/project/p/c777d4a8
@online{al2006link, title={ Correlative SMLM and electron tomography reveals endosome nanoscale domains }, author={ Kalaidzidis, Yannis }, organization={Scitok}, month={ Nov }, day={ 1 }, year={ 2021 }, url = {https://scitok.com/project/p/c777d4a8}, }