Author(s)
Tyler Stephens
Published 4 Projects
Immunology Immune Evasion Structural Rearrangement Type 1 Cr3022
Yaroslav Tsybovsky
Published 9 Projects
COVID-19 Immunology Synthetic Biology Antibody Immune Evasion
Content
A number of broadly neutralizing antibodies (bnAbs) to influenza virus have been isolated, characterized and developed as potential countermeasures for seasonal influenza epidemic and pandemic. Deep characterization of these bnAbs and polyclonal sera is critical to our understanding of influenza immunity and for desgining universal influenza vaccines. However, conventional influenza virus neutralization assays with live viruses require high-containment laboratories and are difficult to standardize and roboticize. Here, we built a panel of engineered influenza viruses carrying a fluorescent reporter gene to replace an essential viral gene. This restricts virus replication to cells expressing the missing viral gene in trans, allowing it to be manipulated in a biosafety level 2 environment. Using this system, we characterize the neutralization profile of a set of published and new bnAbs with a panel consisting of 55 viruses that spans the near complete antigenic evolution of human H1N1 and H3N2 viruses, as well as pandemic viruses such as H5N1 and H7N9. Our system opens opportunities to systematically characterize influenza immunity in greater depth, including the response directed at the viral hemagglutinin stem, a major target of universal influenza vaccines.
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Yaroslav Tsybovsky. (2021, Nov 1).A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies[Video]. Scitok. https://scitok.com/project/p/ce86bd34
Creanga Adrian. "A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies" Scitok, uploaded by Tsybovsky Yaroslav, 1 Nov, 2021, https://scitok.com/project/pce86bd34
Yaroslav Tsybovsky. "A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies" Scitok. (Nov 1, 2021). https://scitok.com/project/p/ce86bd34
Yaroslav Tsybovsky (Nov 1, 2021). A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies Scitok. https://scitok.com/project/p/ce86bd34
Yaroslav Tsybovsky. A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies[video]. 2021 Nov 1. https://scitok.com/project/p/ce86bd34
@online{al2006link, title={ A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies }, author={ Tsybovsky, Yaroslav }, organization={Scitok}, month={ Nov }, day={ 1 }, year={ 2021 }, url = {https://scitok.com/project/p/ce86bd34}, }