Author(s)
Elena G. Govorunova
Published 6 Projects
Biochemistry Biophysics Optogenetics Photobiology Ion Channels
Oleg A. Sineshchekov
Published 6 Projects
Biochemistry Biophysics Optogenetics Photobiology Ion Channels
Content
Video Abstract (AI generated) (00:59) Paper Preprint eLifeThe anion channelrhodopsin GtACR1 from the alga Guillardia theta is a potent neuron-inhibiting optogenetics tool. Presented here, its X-ray structure at 2.9 A reveals a tunnel traversing the protein from its extracellular surface to a large cytoplasmic cavity. The tunnel is lined primarily by small polar and aliphatic residues essential for anion conductance. A disulfide-immobilized extracellular cap facilitates channel closing and the ion path is blocked mid-membrane by its photoactive retinylidene chromophore and further by a cytoplasmic side constriction. The structure also reveals a novel photoactive site configuration that maintains the retinylidene Schiff base protonated when the channel is open. These findings suggest a new channelrhodopsin mechanism, in which the Schiff base not only controls gating, but also serves as a direct mediator for anion flux.
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Oleg A. Sineshchekov. (2021, Nov 3).Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin[Video]. Scitok. https://scitok.com/project/p/d32f27af
Li Hai. "Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin" Scitok, uploaded by A. Sineshchekov Oleg, 3 Nov, 2021, https://scitok.com/project/pd32f27af
Oleg A. Sineshchekov. "Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin" Scitok. (Nov 3, 2021). https://scitok.com/project/p/d32f27af
Oleg A. Sineshchekov (Nov 3, 2021). Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin Scitok. https://scitok.com/project/p/d32f27af
Oleg A. Sineshchekov. Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin[video]. 2021 Nov 3. https://scitok.com/project/p/d32f27af
@online{al2006link, title={ Crystal Structure of a Natural Light-Gated Anion Channelrhodopsin }, author={ A. Sineshchekov, Oleg }, organization={Scitok}, month={ Nov }, day={ 3 }, year={ 2021 }, url = {https://scitok.com/project/p/d32f27af}, }