Author(s)
Elena G. Govorunova
Published 6 Projects
Biochemistry Biophysics Optogenetics Photobiology Ion Channels
Oleg A. Sineshchekov
Published 6 Projects
Biochemistry Biophysics Optogenetics Photobiology Ion Channels
Yumei Wang
Published 3 Projects
Biochemistry Optogenetics Photobiology Ion Channels Author Contributions
Leonid S. Brown
Published 3 Projects
Biochemistry Optogenetics Photobiology Ion Channels Author Contributions
Content
Video Abstract (AI generated) (01:55) Paper Preprint Supplementary MaterialChannelrhodopsins are light-gated ion channels widely used to control neuronal firing with light (optogenetics). We report two previously unknown families of anion channelrhodopsins (ACRs), one from the heterotrophic protists labyrinthulomycetes and the other from haptophyte algae. Four closely related labyrinthulomycete ACRs, named RubyACRs here, exhibit a unique retinal binding pocket that creates spectral sensitivities with maxima at 590-610 nm, the most red-shifted channelrhodopsins known, long-sought for optogenetics, and more broadly the most red-shifted microbial rhodopsins so far reported. We identified three spectral tuning residues critical for the red-shifted absorption. Photocurrents recorded from the RubyACR from Aurantiochytrium limacinum (designated Al ACR1) under single-turnover excitation exhibited biphasic decay, the rate of which was only weakly voltage-dependent, in contrast to that in previously characterized cryptophyte ACRs, indicating differences in channel gating mechanisms between the two ACR families. Moreover, in A. limacinum we identified three ACRs with absorption maxima at 485, 545, and 590 nm, indicating color-sensitive photosensing with blue, green and red spectral variation of ACRs within individual species of the labyrinthulomycete family. We also report energy transfer from a cytoplasmic fluorescent protein domain to the retinal chromophore bound within RubyACRs, not seen in similar constructs in other channelrhodopsins. ### Competing Interest Statement The authors have declared no competing interest.
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Oleg A. Sineshchekov. (2021, Nov 3).RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption[Video]. Scitok. https://scitok.com/project/p/d4d4ca47
G. Govorunova Elena. "RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption" Scitok, uploaded by A. Sineshchekov Oleg, 3 Nov, 2021, https://scitok.com/project/pd4d4ca47
Oleg A. Sineshchekov. "RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption" Scitok. (Nov 3, 2021). https://scitok.com/project/p/d4d4ca47
Oleg A. Sineshchekov (Nov 3, 2021). RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption Scitok. https://scitok.com/project/p/d4d4ca47
Oleg A. Sineshchekov. RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption[video]. 2021 Nov 3. https://scitok.com/project/p/d4d4ca47
@online{al2006link, title={ RubyACRs, non-algal anion channelrhodopsins with highly red-shifted absorption }, author={ A. Sineshchekov, Oleg }, organization={Scitok}, month={ Nov }, day={ 3 }, year={ 2021 }, url = {https://scitok.com/project/p/d4d4ca47}, }