Author(s)
Yannis Kalaidzidis
Published 6 Projects
Cell Biology Pathology Systems Biology Biophysics Bioengineering
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Video Abstract (AI generated) (01:30) Paper PreprintLumen morphogenesis is key to the function of organs and results from the integration of molecular pathways and mechanical forces. The mechanisms governing anisotropic lumen expansion remain elusive. In contrast to epithelial cells which have simple apico-basal polarity and form tubes, hepatocytes are multi-polar and form narrow lumina that grow anisotropically between adjacent cells, collectively generating a complex 3D network of bile canaliculi (BC). Here, we studied lumen elongation and BC morphogenesis in differentiating primary mouse hepatoblasts in vitro. Remarkably, we discovered a pattern of specific extensions of the apical membrane traversing the lumen between adjacent hepatocytes and sealed by tight junctions, reminiscent of the bulkheads of boats. These structures were also present in the developing liver. A targeted screen revealed that silencing of Rab35 caused loss of the bulkheads, conversion of hepatocyte into simple epithelial polarity and formation of spherical lumina in vitro. Strikingly, we could re-engineer hepatocyte polarity and tissue morphogenesis in vivo in the embryonic liver, converting BC into simple epithelial tubes. Our results suggest that the apical bulkheads of hepatocytes are cell-intrinsic anisotropic mechanical elements that ensure stability of the elongating lumen between two cells, thus determining the structure of BC during liver tissue morphogenesis.
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Yannis Kalaidzidis. (2021, Nov 1).Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads[Video]. Scitok. https://scitok.com/project/p/d73084b0
Belicova Lenka. "Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads" Scitok, uploaded by Kalaidzidis Yannis, 1 Nov, 2021, https://scitok.com/project/pd73084b0
Yannis Kalaidzidis. "Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads" Scitok. (Nov 1, 2021). https://scitok.com/project/p/d73084b0
Yannis Kalaidzidis (Nov 1, 2021). Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads Scitok. https://scitok.com/project/p/d73084b0
Yannis Kalaidzidis. Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads[video]. 2021 Nov 1. https://scitok.com/project/p/d73084b0
@online{al2006link, title={ Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads }, author={ Kalaidzidis, Yannis }, organization={Scitok}, month={ Nov }, day={ 1 }, year={ 2021 }, url = {https://scitok.com/project/p/d73084b0}, }