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Video Abstract (AI generated) (01:25) Paper PreprintEndophilins-A are conserved endocytic adaptors with membrane curvature-sensing and -inducing properties. We show here that, independently of their role in endocytosis, endophilin-A1 and endophilin-A2 regulate exocytosis of neurosecretory vesicles. The number of neurosecretory vesicles was not altered in chromaffin cells without endophilin, yet fast capacitance and amperometry measurements revealed reduced exocytosis, smaller vesicle pools and changed fusion kinetics. Both endophilin-A1 (brain-enriched) and A2 (ubiquitous) rescued exocytic defects, but endophilin-A2 was more efficient. Distribution of neurosecretory vesicles was altered in the plasma membrane proximity, but levels and distributions of main exocytic and endocytic factors were unchanged, and slow compensatory endocytosis was not robustly affected. Endophilin's role in exocytosis is mediated through its SH3-domain and, at least in part, interaction with intersectin, a coordinator of exocytic and endocytic traffic. Altogether, we report that endophilins-A, key endocytic proteins linked to neurodegeneration, directly regulate exocytosis by controlling vesicle recruitment, priming and fusion.
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Ira Milosevic. (2021, Nov 4).Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles[Video]. Scitok. https://scitok.com/project/p/ea00a00f
Gowrisankaran Sindhuja. "Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles" Scitok, uploaded by Milosevic Ira, 4 Nov, 2021, https://scitok.com/project/pea00a00f
Ira Milosevic. "Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles" Scitok. (Nov 4, 2021). https://scitok.com/project/p/ea00a00f
Ira Milosevic (Nov 4, 2021). Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles Scitok. https://scitok.com/project/p/ea00a00f
Ira Milosevic. Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles[video]. 2021 Nov 4. https://scitok.com/project/p/ea00a00f
@online{al2006link, title={ Endophilin-A controls recruitment, priming and fusion of neurosecretory vesicles }, author={ Milosevic, Ira }, organization={Scitok}, month={ Nov }, day={ 4 }, year={ 2021 }, url = {https://scitok.com/project/p/ea00a00f}, }