Author(s)
Content
Video Abstract (AI generated) (01:12) Paper PreprintProteoglycans, a class of carbohydrate-modified proteins, often modulate growth factor signaling on the cell surface. However, the molecular mechanism by which proteoglycans regulate signal transduction is largely unknown. In this study, using a recently-developed glycoproteomic method, we found that Windpipe (Wdp) is a novel chondroitin sulfate proteoglycan (CSPG) in Drosophila. Wdp is a single-pass transmembrane protein with leucin-rich repeat (LRR) motifs and bears three CS sugar chain attachment sites in the extracellular domain. Here we show that Wdp modulates the Hedgehog (Hh) pathway. Overexpression of wdp inhibits Hh signaling in the wing disc, which is dependent on its CS chains and the LRR motifs. Conversely, loss of wdp leads to the upregulation of Hh signaling. Furthermore, knockdown of wdp increase the cell surface accumulation of Smoothened (Smo), suggesting that Wdp inhibits Hh signaling by regulating Smo stability. Our study demonstrates a novel role of CSPG in regulating Hh signaling.
More Projects
Loren Frank
13 views • 2 years ago
Global Immunotalks
390 views • 3 years ago
Laurel Yohe
2 views • 2 years ago
Global Immunotalks
130 views • 3 years ago
Jignesh H. Parmar
0 views • 2 years ago
Winston A. Haynes
0 views • 2 years ago
Noam Mazor
0 views • 2 years ago
Global Immunotalks
182 views • 3 years ago
Cem Yuksel
345 views • 3 years ago
Oscar Gonzalez-Recio
3 views • 2 years ago
Please pick a style:
Göran Larson. (2021, Oct 30).Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila[Video]. Scitok. https://scitok.com/project/p/f1af5a48
Takemura Masahiko. "Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila" Scitok, uploaded by Larson Göran, 30 Oct, 2021, https://scitok.com/project/pf1af5a48
Göran Larson. "Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila" Scitok. (Oct 30, 2021). https://scitok.com/project/p/f1af5a48
Göran Larson (Oct 30, 2021). Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila Scitok. https://scitok.com/project/p/f1af5a48
Göran Larson. Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila[video]. 2021 Oct 30. https://scitok.com/project/p/f1af5a48
@online{al2006link, title={ Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila }, author={ Larson, Göran }, organization={Scitok}, month={ Oct }, day={ 30 }, year={ 2021 }, url = {https://scitok.com/project/p/f1af5a48}, }