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We report a novel piece-wise isothermal nucleic acid test (PINAT) for diagnosing pathogen-associated RNA that embeds an exclusive DNA-mediated specific probing reaction with the backbone of an isothermal reverse-transcription cum amplification protocol as a unified single-step procedure. This single step sample-to-result test method has been seamlessly integrated in an inexpensive, scalable, pre-programmable and portable instrument, resulting in a generic platform technology for detecting nucleic acid from a wide variety of pathogens. The test exhibited high sensitivity and specificity of detection of SARS-CoV-2 infection when assessed using 200 double-blind patient samples, conducted by the Indian Council of Medical Research (ICMR), reporting a positive and negative percent agreement of 94.6% and 98% respectively. We also established its efficacy in detecting Influenza-A virus infection, performing the diagnosis at the point of collection with uncompromised detection rigor. The envisaged trade-off between advanced laboratory-based procedures with the elegance of common rapid tests renders the innovation to be ideal for deployment in resource-limited settings towards catering the needs of the underserved.
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Mamta Chawla-Sarkar. (2021, Oct 24).Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration[Video]. Scitok. https://scitok.com/project/p/f755dc07
Banerjee Saptarshi. "Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration" Scitok, uploaded by Chawla-Sarkar Mamta, 24 Oct, 2021, https://scitok.com/project/pf755dc07
Mamta Chawla-Sarkar. "Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration" Scitok. (Oct 24, 2021). https://scitok.com/project/p/f755dc07
Mamta Chawla-Sarkar (Oct 24, 2021). Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration Scitok. https://scitok.com/project/p/f755dc07
Mamta Chawla-Sarkar. Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration[video]. 2021 Oct 24. https://scitok.com/project/p/f755dc07
@online{al2006link, title={ Detecting Pathogen-Associated RNA via Piecewise Isothermal Testing achieving Sample-to-Result Integration }, author={ Chawla-Sarkar, Mamta }, organization={Scitok}, month={ Oct }, day={ 24 }, year={ 2021 }, url = {https://scitok.com/project/p/f755dc07}, }