Author(s)
Shirin Strohmeier
Published 9 Projects
Immunology Microbiology Infectious Diseases Allergy And Immunology Influenza
Fatima Amanat
Published 24 Projects
Infectious Diseases Epidemiology Allergy And Immunology Immunology Covid19
Adolfo GarcĂa-Sastre
Published 9 Projects
COVID-19 Microbiology Biochemistry Infectious Diseases Allergy And Immunology
Lynda Coughlan
Published 5 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Lynda Coughlan
Published 5 Projects
Immunology Microbiology Influenza Hemagglutinin Stalk Universal Vaccine
Content
Video Abstract (AI generated) (01:44) Paper Preprint Emerging Microbes & InfectionsSevere acute respiratory syndrome CoV-2 (SARS-CoV-2) is currently causing a worldwide pandemic with high morbidity and mortality. Development of animal models that recapitulate important aspects of coronavirus disease 2019 (COVID-19) is critical for the evaluation of vaccines and antivirals, and understanding disease pathogenesis. SARS-CoV-2 has been shown to use the same entry receptor as SARS-CoV-1, human angiotensin-converting enzyme 2 (hACE2)(1-3). Due to amino acid differences between murine and hACE2, inbred mouse strains fail to support high titer viral replication of SARS-CoV-2 virus. Therefore, a number of transgenic and knock-in mouse models, as well as viral vector-mediated hACE2 delivery systems have been developed. Here we compared the K18-hACE2 transgenic model to adenovirus-mediated delivery of hACE2 to the mouse lung. We show that K18-hACE2 mice replicate virus to high titers in both the lung and brain leading to lethality. In contrast, adenovirus-mediated delivery results in viral replication to lower titers limited to the lung, and no clinical signs of infection with a challenge dose of 104 plaque forming units. The K18-hACE2 model provides a stringent model for testing the ability of vaccines and antivirals to protect against disease, whereas the adenovirus delivery system has the flexibility to be used across multiple genetic backgrounds and modified mouse strains. ### Competing Interest Statement The authors have declared no competing interest.
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Florian Krammer. (2021, Oct 30).Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection[Video]. Scitok. https://scitok.com/project/p/f7e08c32
Rathnasinghe Raveen. "Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection" Scitok, uploaded by Krammer Florian, 30 Oct, 2021, https://scitok.com/project/pf7e08c32
Florian Krammer. "Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection" Scitok. (Oct 30, 2021). https://scitok.com/project/p/f7e08c32
Florian Krammer (Oct 30, 2021). Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection Scitok. https://scitok.com/project/p/f7e08c32
Florian Krammer. Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection[video]. 2021 Oct 30. https://scitok.com/project/p/f7e08c32
@online{al2006link, title={ Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection }, author={ Krammer, Florian }, organization={Scitok}, month={ Oct }, day={ 30 }, year={ 2021 }, url = {https://scitok.com/project/p/f7e08c32}, }